Analysis Note

ControlBrain, Neuronal culture, Human glioblastoma T98G cells.

Application

Research Sub CategoryNeuronal & Glial Markers

Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.

Research CategoryNeuroscience

Western Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).

Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

Optimal working concentrations must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).

Immunogen

Bovine brain microtubule protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on Rat Brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 µg of Rat Brain lysate.

Specificity

Expected to cross-react with human, cow, chicken, quail, and Xenopus.

MAB3418 binds specifically to MAP-2.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Target description

230 kDa